Abstract

Background Polycystic ovary syndrome PCOS is the most common reproductive endocrinopathy and currently considered to be a part of the metabolic syndrome Women with PCOS cluster risk factors associated with risk of atherosclerosis These risk factors include insulin resistance dyslipidemia hyperandrogenemia obesity endothelial dysfunction and oxidative stress homocysteine and paraoxonase 1 in PCOS in two different regimes of treatment with Metformin and Folic acid in order to elucidate some risk factors that may lead to atherosclerosis and vascular diseases in the corresponding patients Patients and Methods Fifty patients with PCOS were enrolled in this study that subdivided into two groups according to type of treatment received i e G1 comprising 25 patients treated with Metformin only G2 comprisin25 patients with Metformin and Folic acid Serum Homocysteine Hcy Paraoxonase 1 PON1 Apolipoprptein A-1 Apo A-1 and Apolipoprotein B Apo B were determind by using enzymelinked immunosorbent assay ELISA Reduced Glutathion GSH and Malondialdehyde MDA were determined by using colorimetric method Results The results indicated a significant increase in the serum levels of Hcy p 0 01 PON1 and GSH p 0 05 anda significantdecreasein thelevels of MDA and Apo B Apo A-1 ratio p 0 01 in PCOS group after Metformin only G1- A But after Metformin with Folic acid treatment G2-A Hcy Apo B Apo A-1 ratio and MDA levels were significantly lower p 0 01 and PON1 as well as GSH levels were significantly higher p 0 01 in comparison with those before treatment Whereas a significant decreasein level ofHcy MDA p 0 01 and Apo B Apo A-1 ratio p 0 05 anda significantincrease in PON1 p 0 01 and GSH levels p 0 05 in G2-A as compared with G1-A The results revealed a significant positive correlation between Hcywith MDA and Apo B Apo A-1 ratio as well as between PON1 and GSH And a significant negative correlation between HcywithGSH PON1and also betwe