Efficacy of Secondary Metabolites Produced by Bacillus Amyloliquefaciens on the Inhibition of Zymoseptoria Tritici

Barakat Ilham; Chtaina Noureddine; Grappin Philippe; El Guilli Mohemmed; Abdenbi El Hassane; Ezzahiri Brahim

Authors

Barakat Ilham
Chtaina Noureddine
Grappin Philippe
El Guilli Mohemmed
Abdenbi El Hassane
Ezzahiri Brahim

nephelometry, antibiosis, HPLC-MS, secondary metabolites, bacillus amyloliquefaciens I3, zymoseptoria tritici

Abstract

The strain I3 of Bacillus amyloliquefaciens, which was isolated from soft wheat leaves, has revealed, in vitro and in vivo, a high antagonistic potential against septoria leaf blotch of wheat. In order to investigate the existence of antifungal molecules secreted by strain I3, the filtrates of this strain were tested for their inhibitory activity on the germination of pycnidiospores of the two strains of Zymoseptoria tritici, G1-1 and A5-1 isolated from soft wheat and durum wheat, respectively. The antibiosis assays showed a high level of inhibitory activity, with inhibition rates ranging from 94% to 99% compared to the control after 96 hours of incubation. Filtrate analysis by high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS), have identified several families of lipopeptides reported as antifungal molecules (iturins, fengycins, and surfactins); and polyketides (macrolactins, chlorotetaines, and bacillaenes) which would also be responsible for the antagonistic activity against Z. tritici.

Downloads

How to Cite

Efficacy of Secondary Metabolites Produced by Bacillus Amyloliquefaciens on the Inhibition of Zymoseptoria Tritici. (2020). Global Journal of Science Frontier Research, 20(D1), 9-16. https://journalofscience.org/index.php/GJSFR/article/view/2580

Download Citation

References

B Jacobsen (2006) Biological control of plant diseases by phyllosphere applied biological control agents.. 133-147.

Stéphanie Diallo, Alexandre Crépin, Corinne Barbey, Nicole Orange, Jean-François Burini, Xavier Latour (2011) Mechanisms and recent advances in biological control mediated through the potato rhizosphere. 75(3), 351-364.

D Haas, C Keel (2003) Regulation of antibiotic production in root colonizing Pseudomonas spp. and relevance for biological control of plant disease. 41, 117-153.

X Chen, A Koumoutsi, R Scholz, K Schneider, J Vater, R Süssmuth, J Piel, R Borriss (2009) Genome analysis of Bacillus amyloliquefaciens FZB42 reveals its potential for biocontrol of plant pathogens. 140(1-2), 27-37.

Hélène Cawoy, Delphine Debois, Laurent Franzil, Edwin De Pauw, Philippe Thonart, Marc Ongena (2015) Lipopeptides as main ingredients for inhibition of fungal phytopathogens by B acillus subtilis/amyloliquefaciens. 8(2), 281-295.

Karen Kinsella, Cristian Schulthess, Thomas Morris, James Stuart (2009) Rapid quantification of Bacillus subtilis antibiotics in the rhizosphere. 41(2), 374-379.

D Debois, E Jourdan, N Smargiasso, P Thonart, E De Pauw, M Ongena (2014) Spatiotemporal monitoring of the antibiome secreted by Bacillus biofilms on plant roots using MALDI mass spectrometry imaging. 86, 4431-4438.

Hélène Cawoy, Martin Mariutto, Guillaume Henry, Christophe Fisher, Natallia Vasilyeva, Philippe Thonart, Jacques Dommes, Marc Ongena (2014) Plant Defense Stimulation by Natural Isolates of Bacillus Depends on Efficient Surfactin Production. 27(2), 87-100.

Trevor Phister, Daniel O'sullivan, Larry Mckay (2004) Identification of Bacilysin, Chlorotetaine, and Iturin A Produced by Bacillus sp. Strain CS93 Isolated from Pozol, a Mexican Fermented Maize Dough. 70(1), 631-634.

Helene Cawoy, Wagner Bettiol, Patrick Fickers, Marc Onge (2011) Bacillus-Based Biological Control of Plant Diseases. 32.

R Larkin, S Tavantzis (2013) Use of biocontrol organisms and compost amendments for improved control of soilborne diseases and increased potato production. 90, 261-270.

Lili Shen, Fenglong Wang, Yanhua Liu, Yumei Qian, Jinguang Yang, Huiqing Sun (2013) Suppression of Tobacco Mosaic Virus by Bacillus amyloliquefaciens Strain Ba33. 161(4), 293-294.

J Weng, Y Wang, J Li, Q Shen, R Zhang (2012) Enhanced root colonization and biocontrol activity of Bacillus amyloliquefaciens SQR9 by abrB gene disruption. 97, 8823-8830.

I Barakat, N Chtaina, M El Guilli, B Ezzahiri (2018) Evaluation du potentiel antagoniste de quelques isolats de bactéries et de Trichoderma spp.dans le contrôle de Z. tritici agent causal de la septoriose du blé. 6(3), 345-354.

I Barakat, N Chtaina, P Grappin, M El Guilli, B Ezzahiri, S Aligon, M Neveu, M Marchi (2019) Induced Systemic Resistance (ISR) in Arabidopsis thaliana by Bacillus amyloliquefaciens and Trichoderma harzianum Used As Seed Treatments. 9(166), 1-11.

I Barakat, N Chtaina, M El Guilli, B Ezzahiri (2019) Efficacité de Bacillus amyloliquefaciens et Trichoderma harzianum dans le biocontrôle de la septoriose du blé par enrobage des semences. 13, 9-18.

Juvenal Bogoga Kopa, Germain Mabossy-Mobouna, François Malaisse (2017) Le savoir du peuple Budu concernant les chenilles comestibles dans le territoire de Wamba, Haut-Uélé, Répulique Démocratique du Congo. 2(3), 90-100.

Anthony Arguelles-Arias, Marc Ongena, Badre Halimi, Yannick Lara, Alain Brans, Bernard Joris, Patrick Fickers (2009) Bacillus amyloliquefaciens GA1 as a source of potent antibiotics and other secondary metabolites for biocontrol of plant pathogens. 8(1), 1-12.

Lijun Sun, Zhaoxin Lu, Xiaomei Bie, Fengxia Lu, Shengyuan Yang (2006) Isolation and characterization of a co-producer of fengycins and surfactins, endophytic Bacillus amyloliquefaciens ES-2, from Scutellaria baicalensis Georgi. 22(12), 1259-1266.

S Zhang, X Wang, L Meng, J Li, X Zhao, X Cao, X Chen, A Wang, J Li (2012) Isolation and characterization of antifungal lipopeptides produced by endophytic Bacillus amyloliquefaciens TF28. 6(8), 1747-1755.

Ivica Dimkić, Tanja Berić, Tatjana Stević, Snežana Pavlović, Katarina Šavikin, Djordje Fira, Slaviša Stanković (2015) Additive and synergistic effects of Bacillus spp. isolates and essential oils on the control of phytopathogenic and saprophytic fungi from medicinal plants and marigold seeds. 87, 6-13.

Zhihui Xu, Jiahui Shao, Bing Li, Xin Yan, Qirong Shen, Ruifu Zhang (2013) Contribution of Bacillomycin D in Bacillus amyloliquefaciens SQR9 to Antifungal Activity and Biofilm Formation. 79(3), 808-815.

Seung Ji, Narayan Paul, Jian Deng, Young Kim, Bong-Sik Yun, Seung Yu (2013) Biocontrol Activity of Bacillus amyloliquefaciens CNU114001 against Fungal Plant Diseases. 41(4), 234-242.

E Arrebola, R Jacobs, L Korsten (2010) Iturin A is the principal inhibitor in the biocontrol activity ofBacillus amyloliquefaciensPPCB004 against postharvest fungal pathogens. 108(2), 386-395.

Feng-Chia Hsieh, Tsung-Chun Lin, Menghsiao Meng, Suey-Sheng Kao (2008) Comparing Methods for Identifying Bacillus Strains Capable of Producing the Antifungal Lipopeptide Iturin A. 56(1), 1-5.

Philippe Jacques (2011) Surfactin and Other Lipopeptides from Bacillus spp.. 57-91.

K Pathak (2013) Structural Characterization of N-Glycopeptides by Matrix-Dependent Selective Fragmentation of MALDI-TOF/TOF Tandem Mass Spectrometry. 40.

Marc Ongena, Emmanuel Jourdan, Akram Adam, Michel Paquot, Alain Brans, Bernard Joris, Jean‐louis Arpigny, Philippe Thonart (2007) Surfactin and fengycin lipopeptides of Bacillus subtilis as elicitors of induced systemic resistance in plants. 9(4), 1084-1090.

Diego Romero, Antonio De Vicente, Rivo Rakotoaly, Samuel Dufour, Jan-Willem Veening, Eva Arrebola, Francisco Cazorla, Oscar Kuipers, Michel Paquot, Alejandro Pérez-García (2007) The Iturin and Fengycin Families of Lipopeptides Are Key Factors in Antagonism of Bacillus subtilis Toward Podosphaera fusca. 20(4), 430-440.

Jos Raaijmakers, Irene De Bruijn, Ole Nybroe, Marc Ongena (2010) Natural functions of lipopeptides fromBacillusandPseudomonas: more than surfactants and antibiotics. 34(6), 1037-1062.

Harsh Bais, Ray Fall, Jorge Vivanco (2004) Biocontrol ofBacillus subtilisagainst Infection of Arabidopsis Roots byPseudomonas syringaeIs Facilitated by Biofilm Formation and Surfactin Production. 134(1), 307-319.