To develop effective approaches for biotechnological propagation of saffron(Crocus sativus L.)in vitro methods for the induction of morphogenesis and organogenesis were used. Factors influencing morphogenesis and organogenesis of saffron were considered. By using a temperature gradient under in vitro conditions was obtained de novo from 10 to 25 microcorms. Some issues related to biotechnology of saffron were elucidated. The effect of the temperature factor and gibberellin on the efficiency of callus formation and morphogenesis in vitrowerediscussed. The methodical approaches to increase the efficiency of morphogenesis and organogenesis of Crocus sativus L. in in vitro conditions were offered.