Isolation and Characterization of a New Phytotoxic Molecule from Culture Fluids of Verticillium Dahliae
verticillium dahliae, olive tree, new phototoxin, E and Z of cinnamyl acetate., E and Z of cinnamyl acetate
Abstract
The verticillium dahliae liquid culture was extracted with butanol. The BE was able to induce necrosis of cutting stem of olive tree at 40 μg/ml the same toxicity was obtained with acetone precipitate (AP) at 50 μg/ml. The CPG profile of the fraction obtained by purification of BE on preparative TLC showed the presence of two peaks. The identification of these two compounds was based on the mass spectrum and confirmed by the data from library of mass spectra NIST (National Institute of Standards and Technology), and were assigned as two isomers: E-cinnamyl acetate (major product, VdT) and Z-cinnamyl acetate (minor product).
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2013-09-04
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