Genetic Variation of Salt Tolerance Character and Organic Components Studies in Selected Salt Tolerant Genotype and Sensetive Cultivar of Wheat

Abstract

Genetic evaluation was estimated for salt tolerance between the selected wheat genotype through plant breeding program and local cultivar by using random amplified polymorphic DNA (RAPD-PCR reaction) with two selected primers (OP1-01 and GB8). According to the amplification results, the genetic distance, Dendrogram illustrated genetic fingerprint and relationships between the selected genotype (Dijila) and local cultivar (Tamooze-2) were determent under non and saline condition. Also the chlorophyll content and organic compounds of the upper leaves were measured. The amplification results using two primers (OP1-01 and GB8) showed that there are no differences in banding patterns between the selected genotype and local cultivar under non-saline condition, this reflecting that there isn't genetic variation between them under such condition with the two primers which used. Whilst the result showed that there are differences between the selected genotype and local cultivar in their banding patterns with the two primers when grown under saline conditions. Also these results reflecting that there are large genetic variation and distance between the selected genotype and local cultivar in their salt tolerance only under saline condition. The chlorophyll content in the upper leaves of selected genotype increased under saline condition while it decreased in the upper leaves of the local cultivar under saline condition as compared with those under non- saline condition. Also the results indicated that the organic compound in upper leaves increased under saline condition in both genotype and cultivar as compared with those organic were much higher in the selected genotype than local cultivar.