Abstract

A cross-sectional study was conducted from November 2013 to May 2014 on lactating dairy cows to determine the overall prevalence of bovine mastitis identify associated risk factors and isolate the predominant bacterial agents involved in causing mastitis in and around Sinana district A total of 384 lactating cows were examined for mastitis using clinical examination and California Mastitis Test CMT Bacteriological isolation techniques were also undertaken to recover the causative bacterial pathogens Prevalence of mastitis at cow level was 36 72 out of which 4 95 and 31 77 were clinical and subclinical cases respectively The quarter level prevalence was 26 43 from this the clinical and subclinical forms were 2 28 and 24 15 respectively Out of total examined teats 1 30 was blind About 356 bacterial isolates identified from mastitic milk samples The isolates based on their relative frequency of occurrence were Staphylococcus aureus 33 99 Streptococcus agalactiae 24 44 Staphylococcus epidermidis 10 96 Coagulase-Negative Staphylococci CNS 7 58 Escherichia coli 6 46 Streptococcus dysgalactiae 6 18 Corynebacterium bovis 5 34 Klebsiella pneumonia 2 81 and Bacillus cereus 2 23 Risk factors analysis revealed that prevalence of mastitis was significantly differed with the age P 0 01 parity P 0 05 breed p 0 001 stage of lactation p 0 001 mastitis record p 0 01 dry cow therapy p 0 05 udder hygiene p 0 01 drainage system p 0 05 floor type p 0 05 and grazing system P 0 05 Thus prevalence was relatively higher in adult cows OR 1 784 95 CI 0 999 3 189 multiparous cows OR 1 320 95 CI 0 552 3 155 cross breed cows OR 5 820 95 CI 3 248 10 430 early stage lactation OR 3 021 95 CI 1 617 5 647 late stage lactation OR 3 280 95 CI 1 931 5 572 cows with history of mastitis OR 2 452 95 CI 1 282 4 688 cows untreated during drying off OR 1 445 95 CI 0