Establishment of Regeneration Protocol for Canola (Brassica napus L.)
canola, plant regeneration, and ms medium., and ms medium
Abstract
The study was conducted with an aim of developing a genotype independent efficient in vitro regeneration system for Canola (Brassica napus L.) that is not grown in Bangladesh. Plant regeneration was achieved through embryogenic callus production from hypocotyls and cotyledonary leaves with petioles. The optimum medium for callus induction was found with 0.5 mgl-1 2, 4- dichlorophenoxyacetic acid (2, 4-D). The best shooting medium for canola contained 3.0 mgl-1 benzyl amino purine (BAP), 0.1 mgl-1 naphtalene acetic acid (NAA), and 5.0 mgl-1 AgNO3 and produced maximum number of shoots when kinetin was used at a concentration of 0.5 mgl- 1. The profound positive effect of AgNO3 for Canola was found for callogenesis at 0.5 mgl-1 and for regeneration at 5.0 mgl-1. In vitro regenerated shoots of canola produced well developed root system on both the media with 2.5 mgl-1 NAA and 1.0 mgl-1 indole 3-butyric acid (IBA).
Downloads
How to Cite
References
A. Agnihotri, D. Prem, K. Gupta (2004) Biotechnology in Quality improvement of Brassicas, In: Plant Biotechnology & Molecular Markers, Srivastava, P.S., Narula, A and Shila, S (Eds) Anamaya publishers, New Delhi, India. 9, 144-155.
H. Ali, Z. Ali, S. Mehmood, W. Ali (2007) Ali, W., 2007, In vitro regeneration of Brassica napus L cultivars (Star, Cyclone and Westar) from hypocotyls and cotyledonary leaves Pak J. 39, 1251-1256.
BBS (2005) Statistical Pocket Book of Bangladesh.
V. Cardoza, C. Stewart (2003) Increased Agrobacterium-mediated transformation and rooting efficiencies in canola (Brassica napus L.) from hypocotyl segment explants. 21(6), 599-604. https://doi.org/10.1007/s00299-002-0560-y
N. Eskin, B. McDonald (1991) Canola Oil. 16(3), 138-146. https://doi.org/10.1111/j.1467-3010.1991.tb01048.x
J. Ian, Adrian, D. (2003) Comparison of genetically modified and conventionally derived herbicide tolerance in oilseed rape: A case study. 132, 217-226.
M. Khan, H. Rashid, M. Ansar, Z. Chaudry (2003) High frequency shoot regeneration and Agrobacterium-mediated DNA transfer in canola (Brassica napus) Plant Cell Org and Tiss. 75, 223-231.
Toshio Murashige, Folke Skoog (1962) A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. 15(3), 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
P. Salisbery, N. Wratten (1999) Canola In Australia; the first thirty years. 3, 29-36.
J. Williams, D. Pink, N. Biddington (1990) Effect of silver nitrate on long-term culture and regeneration of callus from Brassica oleracea var gemmifera Plant Cell, Tiss and Org. 21(1), 61-66. https://doi.org/10.1007/bf00034493
Published
2013-10-25
Issue
Section
License
Copyright (c) 2013 Authors and Global Journals Private Limited
This work is licensed under a Creative Commons Attribution 4.0 International License.