Degradation of Crude Oil by Bacteria: A Role for Plasmid-Borne Genes
plasmid DNA, crude oil, biodegradation, bacterial isolates, transformation, plasmid curing., plasmid curing
Abstract
The role of plasmid-borne genes in the biodegradation of Chevron Escravos Crude Oil by bacteria was determined. Plasmid extraction and curing, transformation experiments and biodegradation studies were carried out using standard procedures. Plasmid extraction studies showed that two of the six selected crude oil degrading bacterial isolates had two plasmids each. The isolates were Klebsiella pneumoniae from ripe pawpaw fruit and Serratia marscencens from oil palm mill effluent. The plasmids were of small (300bp) and large (>1.5kbp) sizes. The results also showed that the isolates were successfully cured of plasmids using 1% Sodium Dodecyl Sulphate (SDS). The transformation experiment using the extracted plasmid DNA and competent Escherichia coli K12 DH1 cells was successful. The percentage degradation of crude oil at 37oC by E. coli K12 DH1 transformed with the plasmid DNA from Klebsiella pneumoniae was 93.03% while that transformed with the plasmid DNA from Serratia marscencens degraded 76.97% of the crude oil. It was observed that loss of plasmids by Klebsiella pneumoniae and Serratia marscencens did not lead to complete loss of their degradative abilities. It only resulted in reduction in their degradation potential.
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O. Ajayi, J. Ebeigbe (2009) Plasmid profile of bacteria isolated from tears of HIV/AIDS patients. 50(1), 4-8.
A. Akpe (2011) Crude oil degrading capabilities and molecular studies of bacteria isolated from environmental sources in Ekpoma. 124.
R. Barbly, M. Barbour (1984) The degradation of aromatic compounds by the meta and gentisate pathways. In: Microbial Degradation of Organic Compounds. D.T. Gibson (ed) Marcel Dekker, Inc. 253-294.
G. Barrow, Feltham, R. (eds.) (1986) Cowan and Steels Manual for Identification of Medical Bacteria.
M. Benka-Cooker, A. Olumagin (1995) Waste drilling-fluid-utilising microorganisms in a tropical mangrove swamp oilfield location. 53(3), 211-215. https://doi.org/10.1016/0960-8524(95)00055-j
H. Birnboim, J. Doly (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. 7(6), 1513-1523. https://doi.org/10.1093/nar/7.6.1513
D. Brand, S. Lee, B. Yoon (1992) Isolation of a novel pentachloro-phenol-degrading bacterium, Pseudomonas sp. Bu 34. J. Appl. 85, 1-8.
R. Brook, T. Madigan (1991) Biodegradation of low aqueous concentration of pentachlorophenol (PCP) in contaminated groundwater. 3rd (Ed) Laurnet and Pueston Books. 135-141.
L. Caro, G. Chruchwar, M. Chandler (1984) 5 Study of Plasmid Replication in vivo. 17, 72-122.
Z. Chun-Hui, Z. Xiao-Gen, S. Yong-Shu, W. Jian- Hua (2011) Studies on the resistance of extended spectrum beta lactamases. 10(8), 1032-1036.
Andrea Clemente, Tania Anazawa, Lucia Durrant (2001) Biodegradation of polycyclic aromatic hydrocarbons by soil fungi. Bra. J. 32(4), 255-261. https://doi.org/10.1590/s1517-83822001000400001
G. Coral, B. Arikan, M. Unaldi (2006) A preliminary study on tellurite resistance in Pseudomonas species isolated from hospital sewage. Polish J. Environ. 15(3), 517-520.
J. Dillon (1985) Recombinant DNA Methodology, John Willey & Sons. 81-83.
O. Enabulele, A. Orikpete (2009) Extended spectrum B-lactamase production in Gram-negative aerobic isolate from diabetic wounds. Nig. J. 23(1).
F. Esumeh, A. Akpe, O. Eguagie (2009) Crude oil Degrading Capabilities of bacterial isolates from pawpaw (Carica papaya) and sweet orange (Citrus sinensis). A role for plasmid mediated gene. Proceedings of the 1st International Conference, Workshop and exhibition on Biotechnologies for Improved Production of Oil and Gas in the Gulf of Guinea, held in Abuja, Nigeria 1. April 1-3. 2009. 34, 1-7.
D. Focht (1998) Performance of biodegradative microorganisms in soil; xenobiotic chemicals as unexploited metabolic niches, In.: Environmental Biotechnology (ed). Plenum Press. 15-30.
J. Holt (1994) Bergey's Manual of Determinative Bacteriology 9th Edn.
T. Ifeadi, O. Nwankwo (1987) Hydrocarbon mineralization in relation bacterial and fungal contribution. Soil Biol. 2, 56-58.
U. Ijah, S. Antai (2003) The Potential Use of Chicken-Drop Micro-Organisms for Oil Spill Remediation. 23(1), 89-95. https://doi.org/10.1023/a:1022947727324
V. Lexmi, S. Padma, H. Polasa (1987) Elimination of multidrug resistance plasmid in bacteria by Plumbagin derived from plants. Curr. 16, 24.
N. McClune, Weightman J, J. Fry (1989) Survival of Pseudomonas putida UWC1 containing cloned catabolic genes in a model activated-sludge unit. 55(10), 2627-2634. https://doi.org/10.1128/aem.55.10.2627-2634.1989
T. Mandri, J. Lin (2007) Isolation and characterization of engine oil degrading indigenous microorganisms in Kwazulu-Natal. Afr. J. 6(1), 23-27.
S. Mervat (2009) Degradation of methomyl by the novel bacterial strain Stenotrophomonasmaltophilia M1. Elect. J. 12(4), 1-6.
A. Mills, C. Brenil, R. Colwell (1978) Enumeration of petroleum degrading marine and estuarine microorganisms by most probable number method. 24(5), 552-557. https://doi.org/10.1139/m78-089
Sanjeet Mishra, Jeevan Jyot, Ramesh Kuhad, Banwari Lal (2001) Evaluation of inoculum addition to stimulate in-situ bioremediation of oily-sludge-contaminated soil. Appl. Environ. 67(4), 1675-1681. https://doi.org/10.1128/aem.67.4.1675-1681.2001
A. Nelson (1990) The biodegradation of halogenated organic compounds. J. Appl. 69, 445-470.
I. Okoh, S. Ajisebutu, G. Babalola, M. Trejo- Hermandez (2001) Potential of Burkholderia cepacia RQ1 in the biodegradation of heavy crude oil. 4(2), 83-87. https://doi.org/10.1007/s101230100018
G. Okpokwasili, S. Amanchukwu (1988) Petroleum hydrocarbon degradation by Candida species. 14(3), 243-247. https://doi.org/10.1016/0160-4120(88)90145-6
N. Oruansi, C. Ogugbue (2003) Effect of ph and nutrient starvation on biodegradation of azo dyes by Cellulomonas sp. 6(1), 58-63. https://doi.org/10.4314/ajazeb.v6i1.41176
S. Philips, D. Dalgarn, S. Young (1989) Recombinant DNA in wastewater. pB 322 degradation kinetics. J. Water Pollut. Contr. 61, 1588-1595.
J. Sambrook, E. Fritsch, T. Maniatis (1989) Molecular cloning: laboratory manual, 2nd ed. 0-8796.
R. Shoemaker (1989) Exploitation of indigenous microorganisms for the clean-up of oil spills. 25, 51-52.
A. Stanisich (1984) 2 Identification and Analysis of Plasmids at the Genetic Level. 17, 5-32. https://doi.org/10.1016/s0580-9517(09)70050-9
H. Vahaboglon, S. Dodanli, R. Ozturk (1996) Characterization of multiple antibiotic resistant Salmonella typhimurium strains, molecular epidemiology of PCR-1-producing isolates and evidence for nosocomial plasmid exchange by a clone. 34(12), 2942-2946. https://doi.org/10.1128/jcm.34.12.2942-2946.1996
U. Winkler, W. Ruger, L. Wackernagew (1979) Bacterial, Phage and Molecular Genetics. 127-128.
G. Zylstra, D. Gibson (1991) Aromatic hydrocarbon Degradation: A Molecular Approach. Genet. 13, 183-203.
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