Storage Response of Neem (Azadirachta Indica A. Juss.) Seed under Different Moisture and Temperature Regime

Devendra Kumar

Authors

Devendra Kumar

germination percentage, mean germination time (MGT), germination value (GV), azadirachta indica, viability

Abstract

Effect of four storage temperatures (T1-ambient room temperature (35±5ºC), T2-Air conditioner (25±2ºC), T3- Fridge (5±1ºC) and T4-Freezer (-4±1ºC) and four moisture levels M1 (38%), M2 (21%), M3 (12%), M4 (5.5%) were studied on seed longevity of seeds under controlled conditions. After setting of desired moisture content seeds were then subjected to storage treatments in 200µ thick polythene bag and kept in airtight plastic container at different temperature levels and tested upto 270 days respectively. Seeds were sown in between paper (BP) for germination study in laboratory at 30±1ºC and relative humidity (90±3%). Freshly collected seeds after drying at target levels showed germination from 93 to 96.5 %. Seeds having M1, M2 and M3 moisture content completely lost germination well before 90 days at T1 temperature. Seeds of M4 moisture level showed 55.37% decline in germination upto 90 days. Seeds containing moisture M1, M2, M3 and M4 at T2 and T3 level showed 100%, 56.5%, 14.74%, 5.21% and 100%, 18.14%, 11.58%, 6.25% respectively decline in germination upto 90 days of storage. Seeds (M4) stored at T4 level showed 20.83% reduction in seed germination. After 150 days of seed storage, the rate of decline was observed higher in T1 at all level of moistures, T2 at M1 (100%), M2 (64.29%), M3 (8.64%), M4 (5.69%) and in T3 at M1 (100%), M2 (8.61%), M3 (16.67%), M4 (8.89%). Rate of decline in germination percentage was lower at T4 temperature and M4 (3.46%) moisture level between these two periods. Seeds stored at low temperature and moisture content (T3M4) exhibited greater mean viability period (P50, 326 days) as compared to the seeds stored at higher temperature and moisture. However, P50 was not increased when seeds were stored at lower temperature and moisture content (T4M4, 184 days). Thus present finding reveals that seeds of neem may be stored at 5±1ºC (T3) temperature with moisture 5.5% (M4) for 326 days (P50) and data also indicate the orthodox behaviour of neem seed of this region.

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Storage Response of Neem (Azadirachta Indica A. Juss.) Seed under Different Moisture and Temperature Regime. (2015). Global Journal of Science Frontier Research, 15(48), 5-17. https://journalofscience.org/index.php/GJSFR/article/view/

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References

R. Bellefontaine, M. Audinet (1993) La conservation de graines de neem (Azadirachta indica A Juss.) In: Some, L.M and De Kam, M. 268-274.

P. Berjak, D. Dumet (1996) Cryopreservation of seeds and isolated embryonic axes of Neem (Azadirachta indica). 17, 99-104.

P. Berjak, G. Campbell, J. Farrant, W. Omandi- Oloo, N. Pammenter (1995) Responses of seeds of Azadirachta indica (neem) to short-term storage under ambient or chilled conditions. 23, 779-792.

K. Chaisurisri, B. Ponoy, P. Wasuwanich (1986) Storage of Azadirachta indica A. Juss. Seeds. 1, 19-27.

W. Chaney, D. Knudson (1988) Germination of seeds of Azadirachta indica enhanced by endocarp removal. 5, 230-233.

H. Chin, E. Roberts (1980) Recalcitrant crop seeds.

H. Chin, B. Krisinapillay, P. Stanwood (1989) Seed moisture recalcitrant vs. orthodox seeds. 5-22.

F. Corbineau, D. Come (1988) Storage of recalcitrant seeds of four tropical species. 16, 97-103.

J. Crowe, F. Hoekstra, I. Crowe, T. Anchordologuy, E. Dorbnis (1989) Lipid phase transitions measured in intact cells with Fourier transform infrared spectroscopy. 26(1), 76-84. https://doi.org/10.1016/0011-2240(89)90035-7

J. Dickie, R. Smith (1992) Limits to the survival of essentially orthodox seeds at low moisture contents in some woody species.

K. Djavanshir, H. Pourbeik (1976) Germination value -a new formula. 25, 79-83.

R. Ellis, T. Hong, E. Roberts (1990) 1990a An intermediate category of seed storage behaviour of Coffee. 41(9), 1167-1174. https://doi.org/10.1093/jxb/41.9.1167

R. Ellis, T. Hong, E. Roberts (1990) Moisture Content and the Longevity of Seeds of Phaseolus vulgaris. 66(3), 341-348. https://doi.org/10.1093/oxfordjournals.aob.a088033

R. Ellis, T. Hong, E. Roberts (1991) An intermediate category of seed storage behaviour II. Effects of provenance, immaturity and imbibition on desiccation-tolerance in coffee. 42(5), 653-657. https://doi.org/10.1093/jxb/42.5.653

B. Ezumah (1986) Germination of storage of neem (Azadirachta indica A. Juss.). 14, 593-600.

D. Finney (1952) Plant Analysis.

C. Gamene, H. Kraak, J. Pijlen (1994) Azadirachta indica seeds from Burkina Faso: intermediate storage behaviour. 23.

C. Gamene, H. Kraak, J. Van Pijlen, C. De Vos (1996) Storage behaviour of neem (A. indica) seeds from Burkino Faso. 24, 441-448.

D. Graham, B. Patterson (1982) Responses of plants to low, non-freezing temperatures: proteins, metabolism and acclimation. 33(1), 347-372. https://doi.org/10.1146/annurev.pp.33.060182.002023

J. Hanson (1984) The storage of seeds of tropical fruits. 53-62.

(1993) International Rules for Seed Testing. 21.

M. King, E. Roberts (1979) The storage of recalcitrant seedachievements and possible approaches.

H. Kraak (1993) Physiological aspects of storage of recalcitrant seeds. 239-253.

Devendra Kumar, D. Mishra, B. Singh, V. Singh (2008) Effect of methods of drying on viability, storability and seedling performance of Neem (Azadirachta indica A. Juss.) seeds. 134, 991-1002.

Devendra Kumar, D. Mishra, B. Singh (2007) Effect of temperature, media and light on germination of Neem (Azadirachta indica A. Juss.) seeds. 133, 1636-1642.

Devendra Kumar, D. Mishra, R. Singh (2007) Effect of different methods of seed extraction on viability, storability and seedling performance of neem (Azadirachta indica A. Juss.) seeds. 43, 129-138.

Devendra Kumar, D. Mishra, S. Sharma (2009) A simplified package of seed processing of important agro forestry species (Azadirachta indica A. Juss.) for forestry field personals. 82-90.

Kumar Devendra, D. Mishra (2007) Effect of methods of seed collection on seed qualities and storability of Azadirachta indica A. Juss. (Neem) seed. 14, 271-276.

Kumar Devendra, D. Mishra (2007) Effect of fruits colour on germination, storability, and seedling performance in Neem (Azadirachta indica A. Juss.) seed. 14, 209-215.

J. Lyons, J. Raison, S. Steponkus (1979) The plant membrane in response to low temperature: an overview. 1-24.

G. Maithani, V. Bahuguna, M. Rawat, O. Sood (1989) Fruit maturity and interrelated effects of temperatures and container on longevity of neem (Azadirachta indica) seed. 115, 89-97.

N. Pammenter, Patricia Berjak (1999) A review of recalcitrant seed physiology in relation to desiccation-tolerance mechanisms. 9(1), 13-37. https://doi.org/10.1017/s0960258599000033

K. Platt-Alota, W. Thomson (1987) Freeze fracture evidence for lateral phase separations in the plasmalemma of chilling-injured avocado fruit. 136(2-3), 71-80. https://doi.org/10.1007/bf01276356

A. Ponnuswamay, R. Vinaya Rai, C. Surendran, T. Karivaratharaju (1990) Studies on maintaining seed longevity and the effect of fruit grades in neem (Azadirachta indica). 3, 285-290.

M. Rawat, R. Thapliyal (2003) Endogenous rhythm in seed germination of Dendrocalamus strictus. 31(1), 21-27. https://doi.org/10.15258/sst.2003.31.1.03

E. Roberts (1972) Storage Environment and the Control of Viability. 14-58. https://doi.org/10.1007/978-94-009-5685-8

E. Roberts (1973) Predicting the storage life of seeds. 1, 499-514.

E. Roberts, R. Ellis (1989) Water and seed survival. 63, 39-52.

E. Roberts, M. King, R. Ellis (1984) Recalcitrant seedstheir recognition and storage In: Holden, J.H.W. 38-52.

Y. Roederer, R. Bellefontaine (1989) Can Neem seed be expected to keep their germination capacity for several years after collection?. 17, 30-33.

M. Sacande, J. Van Pijlen, C. De Vos, F. Hoekstra, R. Bino, S. Groot (1996) Intermediate storage behaviour of neem tree (Azadirachta indica) seeds from Burkino Faso. 101-104.

M. Sacande, Folkert A (1998) A multifactorial study of conditions influencing longevity of neem (Azadirachta indica) seeds. 8(4), 473-482. https://doi.org/10.1017/s096025850000444x

M. Sacande, F. Hoekstra, K. Khoo, N. Jayanthi, F. Tsan, Krishnapillay (1998) Recalcitrant seeds. 1999, 12-15.

D. Tiwari (1992) Monograph on Neem (Azadirachta indica A. Juss).

P. Tompsett (1994) Capture of genetic resources by collection and storage of seeds: a physiological approach. 61-71.

C. Vertucci, E. Roos, J. Crane (1994) Theoretical Basis of Protocols for Seed Storage III. Optimum Moisture Contents for Pea Seeds Stored at Different Temperatures. 74(5), 531-540. https://doi.org/10.1006/anbo.1994.1151

Chien Wang (1982) Physiological and biochemical responses of plants to chilling stress. 17(2), 173-186. https://doi.org/10.21273/hortsci.17.2.173

R. Willan (1985) A guide to Forest seed handling with special reference to the tropics.

Kazuki Yamawaki, Naoki Yamauchi, Kazuo Chachin, Takashi Iwata (1983) Relationship of Mitochondrial Enzyme Activity to Chilling Injury of Cucumber Fruit. 52(1), 93-98. https://doi.org/10.2503/jjshs.52.93

Shizuo Yoshida, Takefumi Kawata, Matsuo Uemura, Teruo Niki (1986) Properties of Plasma Membrane Isolated from Chilling-Sensitive Etiolated Seedlings of Vigna radiata L. 80(1), 152-160. https://doi.org/10.1104/pp.80.1.152