This study was conducted with the aim of isolating and identifying novel strains of Bacillus thuringiensis (Bt) from the three different kinds of soils and to determine its antibiotic susceptibility. Soil samples were taken from three different uncultivated sites that have no history of treatment with Bt products. Isolation of the Bt strain was conducted according to the method as described by Travers et al (1987) and the phenotypic characteristics of the isolated Bt were determined by making use of standard methods. Twenty presumptively identified strains of Bt were subjected to antibiotic testing by using ampicillin, amoxicillin, tetracycline, streptomycin, and ofloxacin. Results showed that the isolated strain of Bt were resistant to Î²-lactams (amoxicillin and ampicillin).